Publications

1: Machlus KR, Thon JN, Italiano JE Jr. Interpreting the developmental dance of the megakaryocyte: a review of the cellular and molecular processes mediating platelet formation. Br J Haematol. 2014 Apr;165(2):227-36. doi: 10.1111/bjh.12758. Epub 2014 Feb 6. PubMed PMID: 24499183.

2: Battinelli EM, Markens BA, Kulenthirarajan RA, Machlus KR, Flaumenhaft R, Italiano JE Jr. Anticoagulation inhibits tumor cell-mediated release of platelet angiogenic proteins and diminishes platelet angiogenic response. Blood. 2014 Jan 2;123(1):101-12. doi: 10.1182/blood-2013-02-485011. Epub 2013 Sep 24. PubMed PMID: 24065244; PubMed Central PMCID: PMC3879901.

3: Hildick-Smith GJ, Cooney JD, Garone C, Kremer LS, Haack TB, Thon JN, Miyata N, Lieber DS, Calvo SE, Akman HO, Yien YY, Huston NC, Branco DS, Shah DI, Freedman ML, Koehler CM, Italiano JE Jr, Merkenschlager A, Beblo S, Strom TM, Meitinger T, Freisinger P, Donati MA, Prokisch H, Mootha VK, DiMauro S, Paw BH. Macrocytic anemia and mitochondriopathy resulting from a defect in sideroflexin 4. Am J Hum Genet. 2013 Nov 7;93(5):906-14. doi: 10.1016/j.ajhg.2013.09.011. Epub 2013 Oct 10. PubMed PMID: 24119684; PubMed Central PMCID: PMC3824126.

4: Thon JN, Kitterman AC, Italiano JE Jr. Animating platelet production adds physiological context. Trends Mol Med. 2013 Oct;19(10):583-5. doi: 10.1016/j.molmed.2013.07.010. Epub 2013 Aug 14. PubMed PMID: 23953478.

5: Gobbi G, Mirandola P, Carubbi C, Masselli E, Sykes SM, Ferraro F, Nouvenne A, Thon JN, Italiano JE Jr, Vitale M. Proplatelet generation in the mouse requires PKCε-dependent RhoA inhibition. Blood. 2013 Aug 15;122(7):1305-11. doi: 10.1182/blood-2013-04-490599. Epub 2013 Jul 9. PubMed PMID: 23838351.

6: Machlus KR, Italiano JE Jr. The incredible journey: From megakaryocyte development to platelet formation. J Cell Biol. 2013 Jun 10;201(6):785-96. doi: 10.1083/jcb.201304054. Review. PubMed PMID: 23751492; PubMed Central PMCID: PMC3678154.

7: Koseoglu S, Dilks JR, Peters CG, Fitch-Tewfik JL, Fadel NA, Jasuja R, Italiano JE Jr, Haynes CL, Flaumenhaft R. Dynamin-related protein-1 controls fusion pore dynamics during platelet granule exocytosis. Arterioscler Thromb Vasc Biol. 2013 Mar;33(3):481-8. doi: 10.1161/ATVBAHA.112.255737. Epub 2013 Jan 3. PubMed PMID: 23288151; PubMed Central PMCID: PMC3573216.

8: Italiano JE Jr. Unraveling mechanisms that control platelet production. Semin Thromb Hemost. 2013 Feb;39(1):15-24. doi: 10.1055/s-0032-1331157. Epub 2012 Dec 24. Review. PubMed PMID: 23266965.

9: Shimizu A, Nakayama H, Wang P, König C, Akino T, Sandlund J, Coma S, Italiano JE Jr, Mammoto A, Bielenberg DR, Klagsbrun M. Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding protein. J Biol Chem. 2013 Jan 25;288(4):2210-22. doi: 10.1074/jbc.M112.397398. Epub 2012 Nov 29. PubMed PMID: 23195957; PubMed Central PMCID: PMC3554894.

10: Macaulay IC, Thon JN, Tijssen MR, Steele BM, MacDonald BT, Meade G, Burns P, Rendon A, Salunkhe V, Murphy RP, Bennett C, Watkins NA, He X, Fitzgerald DJ, Italiano JE Jr, Maguire PB. Canonical Wnt signaling in megakaryocytes regulates proplatelet formation. Blood. 2013 Jan 3;121(1):188-96. doi: 10.1182/blood-2012-03-416875. Epub 2012 Nov 16. PubMed PMID: 23160460; PubMed Central PMCID: PMC3538329.

11: Begonja AJ, Gambaryan S, Schulze H, Patel-Hett S, Italiano JE Jr, Hartwig JH, Walter U. Differential roles of cAMP and cGMP in megakaryocyte maturation and platelet biogenesis. Exp Hematol. 2013 Jan;41(1):91-101.e4. doi: 10.1016/j.exphem.2012.09.001. Epub 2012 Sep 11. PubMed PMID: 22981933; PubMed Central PMCID: PMC3638753.

12: Nürnberg ST, Rendon A, Smethurst PA, Paul DS, Voss K, Thon JN, Lloyd-Jones H, Sambrook JG, Tijssen MR; HaemGen Consortium, Italiano JE Jr, Deloukas P, Gottgens B, Soranzo N, Ouwehand WH. A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in megakaryocytes near a MEIS1 binding site. Blood. 2012 Dec 6;120(24):4859-68. doi: 10.1182/blood-2012-01-401893. Epub 2012 Sep 12. PubMed PMID: 22972982; PubMed Central PMCID: PMC3520622.

13: Meyer I, Kunert S, Schwiebert S, Hagedorn I, Italiano JE Jr, Dütting S, Nieswandt B, Bachmann S, Schulze H. Altered microtubule equilibrium and impaired thrombus stability in mice lacking RanBP10. Blood. 2012 Oct 25;120(17):3594-602. doi: 10.1182/blood-2012-01-401737. Epub 2012 Aug 30. PubMed PMID: 22936655.

14: Drabek K, Gutiérrez L, Vermeij M, Clapes T, Patel SR, Boisset JC, van Haren J, Pereira AL, Liu Z, Akinci U, Nikolic T, van Ijcken W, van den Hout M, Meinders M, Melo C, Sambade C, Drabek D, Hendriks RW, Philipsen S, Mommaas M, Grosveld F, Maiato H, Italiano JE Jr, Robin C, Galjart N. The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance. Cell Rep. 2012 Oct 25;2(4):781-8. doi: 10.1016/j.celrep.2012.08.040. Epub 2012 Oct 19. PubMed PMID: 23084744.

15: Smith EC, Thon JN, Devine MT, Lin S, Schulz VP, Guo Y, Massaro SA, Halene S, Gallagher P, Italiano JE Jr, Krause DS. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood. 2012 Sep 13;120(11):2317-29. doi: 10.1182/blood-2012-04-420828. Epub 2012 Jul 17. PubMed PMID: 22806889; PubMed Central PMCID: PMC3447785.

16: Thon JN, Devine MT, Jurak Begonja A, Tibbitts J, Italiano JE Jr. High-content live-cell imaging assay used to establish mechanism of trastuzumab emtansine (T-DM1)–mediated inhibition of platelet production. Blood. 2012 Sep 6;120(10):1975-84. doi: 10.1182/blood-2012-04-420968. Epub 2012 Jun 4. PubMed PMID: 22665936; PubMed Central PMCID: PMC3437592.

17: Thon JN, Italiano JE Jr. Does size matter in platelet production? Blood. 2012 Aug 23;120(8):1552-61. doi: 10.1182/blood-2012-04-408724. Epub 2012 Jun 4. Review. PubMed PMID: 22665937; PubMed Central PMCID: PMC3429301. 18: Thon JN, Peters CG, Machlus KR, Aslam R, Rowley J, Macleod H, Devine MT, Fuchs TA, Weyrich AS, Semple JW, Flaumenhaft R, Italiano JE Jr. T granules in human platelets function in TLR9 organization and signaling. J Cell Biol. 2012 Aug 20;198(4):561-74. doi: 10.1083/jcb.201111136. PubMed PMID: 22908309; PubMed Central PMCID: PMC3514030.

19: Wandall HH, Rumjantseva V, Sørensen AL, Patel-Hett S, Josefsson EC, Bennett EP, Italiano JE Jr, Clausen H, Hartwig JH, Hoffmeister KM. The origin and function of platelet glycosyltransferases. Blood. 2012 Jul 19;120(3):626-35. doi: 10.1182/blood-2012-02-409235. Epub 2012 May 21. PubMed PMID: 22613794; PubMed Central PMCID: PMC3401214.

20: Peterson JE, Zurakowski D, Italiano JE Jr, Michel LV, Connors S, Oenick M, D’Amato RJ, Klement GL, Folkman J. VEGF, PF4 and PDGF are elevated in platelets of colorectal cancer patients. Angiogenesis. 2012 Jun;15(2):265-73. doi: 10.1007/s10456-012-9259-z. Epub 2012 Mar 9. PubMed PMID: 22402885.

21: Thon JN, Macleod H, Begonja AJ, Zhu J, Lee KC, Mogilner A, Hartwig JH, Italiano JE Jr. Microtubule and cortical forces determine platelet size during vascular platelet production. Nat Commun. 2012 May 22;3:852. doi: 10.1038/ncomms1838. PubMed PMID: 22617292.

22: Patel-Hett S, Wang H, Begonja AJ, Thon JN, Alden EC, Wandersee NJ, An X, Mohandas N, Hartwig JH, Italiano JE Jr. The spectrin-based membrane skeleton stabilizes mouse megakaryocyte membrane systems and is essential for proplatelet and platelet formation. Blood. 2011 Aug 11;118(6):1641-52. doi: 10.1182/blood-2011-01-330688. Epub 2011 May 12. PubMed PMID: 21566095; PubMed Central PMCID: PMC3156050.

23: Battinelli EM, Markens BA, Italiano JE Jr. Release of angiogenesis regulatory proteins from platelet alpha granules: modulation of physiologic and pathologic angiogenesis. Blood. 2011 Aug 4;118(5):1359-69. doi: 10.1182/blood-2011-02-334524. Epub 2011 Jun 16. PubMed PMID: 21680800; PubMed Central PMCID: PMC3152500.

24: Semple JW, Italiano JE Jr, Freedman J. Platelets and the immune continuum. Nat Rev Immunol. 2011 Apr;11(4):264-74. doi: 10.1038/nri2956. Review. PubMed PMID: 21436837.

25: Thon JN, Montalvo A, Patel-Hett S, Devine MT, Richardson JL, Ehrlicher A, Larson MK, Hoffmeister K, Hartwig JH, Italiano JE Jr. Cytoskeletal mechanics of proplatelet maturation and platelet release. J Cell Biol. 2010 Nov 15;191(4):861-74. doi: 10.1083/jcb.201006102. PubMed PMID: 21079248; PubMed Central PMCID: PMC2983072.

26: Italiano JE Jr, Mairuhu AT, Flaumenhaft R. Clinical relevance of microparticles from platelets and megakaryocytes. Curr Opin Hematol. 2010 Nov;17(6):578-84. doi: 10.1097/MOH.0b013e32833e77ee. Review. PubMed PMID: 20739880; PubMed Central PMCID: PMC3082287.

27: Halene S, Gao Y, Hahn K, Massaro S, Italiano JE Jr, Schulz V, Lin S, Kupfer GM, Krause DS. Serum response factor is an essential transcription factor in megakaryocytic maturation. Blood. 2010 Sep 16;116(11):1942-50. doi: 10.1182/blood-2010-01-261743. Epub 2010 Jun 4. PubMed PMID: 20525922; PubMed Central PMCID: PMC3173990.

28: Peterson JE, Zurakowski D, Italiano JE Jr, Michel LV, Fox L, Klement GL, Folkman J. Normal ranges of angiogenesis regulatory proteins in human platelets. Am J Hematol. 2010 Jul;85(7):487-93. doi: 10.1002/ajh.21732. PubMed PMID: 20575035.

29: Zaslavsky A, Baek KH, Lynch RC, Short S, Grillo J, Folkman J, Italiano JE Jr, Ryeom S. Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis. Blood. 2010 Jun 3;115(22):4605-13. doi: 10.1182/blood-2009-09-242065. Epub 2010 Jan 19. PubMed PMID: 20086246; PubMed Central PMCID: PMC2881490.

30: Chase TH, Lyons BL, Bronson RT, Foreman O, Donahue LR, Burzenski LM, Gott B, Lane P, Harris B, Ceglarek U, Thiery J, Wittenburg H, Thon JN, Italiano JE Jr, Johnson KR, Shultz LD. The mouse mutation “thrombocytopenia and cardiomyopathy” (trac) disrupts Abcg5: a spontaneous single gene model for human hereditary phytosterolemia/sitosterolemia. Blood. 2010 Feb 11;115(6):1267-76. doi: 10.1182/blood-2009-05-219808. Epub 2009 Oct 21. PubMed PMID: 19846887; PubMed Central PMCID: PMC2826237.

31: Italiano JE Jr, Battinelli EM. Selective sorting of alpha-granule proteins. J Thromb Haemost. 2009 Jul;7 Suppl 1:173-6. doi: 10.1111/j.1538-7836.2009.03387.x. Review. PubMed PMID: 19630794; PubMed Central PMCID: PMC3004150.

32: Flaumenhaft R, Dilks JR, Richardson J, Alden E, Patel-Hett SR, Battinelli E, Klement GL, Sola-Visner M, Italiano JE Jr. Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. Blood. 2009 Jan 29;113(5):1112-21. doi: 10.1182/blood-2008-06-163832. Epub 2008 Sep 18. PubMed PMID: 18802008; PubMed Central PMCID: PMC2635076.

33: Shimizu A, Mammoto A, Italiano JE Jr, Pravda E, Dudley AC, Ingber DE, Klagsbrun M. ABL2/ARG tyrosine kinase mediates SEMA3F-induced RhoA inactivation and cytoskeleton collapse in human glioma cells. J Biol Chem. 2008 Oct 3;283(40):27230-8. doi: 10.1074/jbc.M804520200. Epub 2008 Jul 25. PubMed PMID: 18660502; PubMed Central PMCID: PMC2555994.

34: Schulze H, Dose M, Korpal M, Meyer I, Italiano JE Jr, Shivdasani RA. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. J Biol Chem. 2008 May 16;283(20):14109-19. doi: 10.1074/jbc.M709397200. Epub 2008 Mar 17. PubMed PMID: 18347012; PubMed Central PMCID: PMC2376235.

35: Patel-Hett S, Richardson JL, Schulze H, Drabek K, Isaac NA, Hoffmeister K, Shivdasani RA, Bulinski JC, Galjart N, Hartwig JH, Italiano JE Jr. Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules. Blood. 2008 May 1;111(9):4605-16. doi: 10.1182/blood-2007-10-118844. Epub 2008 Jan 29. PubMed PMID: 18230754; PubMed Central PMCID: PMC2343595.

36: Italiano JE Jr, Richardson JL, Patel-Hett S, Battinelli E, Zaslavsky A, Short S, Ryeom S, Folkman J, Klement GL. Angiogenesis is regulated by a novel mechanism: pro- and antiangiogenic proteins are organized into separate platelet alpha granules and differentially released. Blood. 2008 Feb 1;111(3):1227-33. Epub 2007 Oct 25. PubMed PMID: 17962514; PubMed Central PMCID: PMC2214735.

37: Cervi D, Yip TT, Bhattacharya N, Podust VN, Peterson J, Abou-Slaybi A, Naumov GN, Bender E, Almog N, Italiano JE Jr, Folkman J, Klement GL. Platelet-associated PF-4 as a biomarker of early tumor growth. Blood. 2008 Feb 1;111(3):1201-7. Epub 2007 Oct 3. PubMed PMID: 17914028.

38: Junt T, Schulze H, Chen Z, Massberg S, Goerge T, Krueger A, Wagner DD, Graf T, Italiano JE Jr, Shivdasani RA, von Andrian UH. Dynamic visualization of thrombopoiesis within bone marrow. Science. 2007 Sep 21;317(5845):1767-70. PubMed PMID: 17885137.

39: Battinelli EM, Hartwig JH, Italiano JE Jr. Delivering new insight into the biology of megakaryopoiesis and thrombopoiesis. Curr Opin Hematol. 2007 Sep;14(5):419-26. Review. PubMed PMID: 17934346.

40: Italiano JE Jr, Patel-Hett S, Hartwig JH. Mechanics of proplatelet elaboration. J Thromb Haemost. 2007 Jul;5 Suppl 1:18-23. Review. PubMed PMID: 17635704.

41: Hartwig JH, Italiano JE Jr. Cytoskeletal mechanisms for platelet production. Blood Cells Mol Dis. 2006 Mar-Apr;36(2):99-103. Epub 2006 Feb 7. PubMed PMID: 16464622.

42: Patel SR, Richardson JL, Schulze H, Kahle E, Galjart N, Drabek K, Shivdasani RA, Hartwig JH, Italiano JE Jr. Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes. Blood. 2005 Dec 15;106(13):4076-85. Epub 2005 Aug 23. PubMed PMID: 16118321; PubMed Central PMCID: PMC1895246.

43: Richardson JL, Shivdasani RA, Boers C, Hartwig JH, Italiano JE Jr. Mechanisms of organelle transport and capture along proplatelets during platelet production. Blood. 2005 Dec 15;106(13):4066-75. Epub 2005 Aug 23. PubMed PMID: 16118320; PubMed Central PMCID: PMC1895242.

44: Patel SR, Hartwig JH, Italiano JE Jr. The biogenesis of platelets from megakaryocyte proplatelets. J Clin Invest. 2005 Dec;115(12):3348-54. Review. PubMed PMID: 16322779; PubMed Central PMCID: PMC1297261.

45: Schulze H, Korpal M, Bergmeier W, Italiano JE Jr, Wahl SM, Shivdasani RA. Interactions between the megakaryocyte/platelet-specific beta1 tubulin and the secretory leukocyte protease inhibitor SLPI suggest a role for regulated proteolysis in platelet functions. Blood. 2004 Dec 15;104(13):3949-57. Epub 2004 Aug 17. PubMed PMID: 15315966.

46: Tiwari S, Italiano JE Jr, Barral DC, Mules EH, Novak EK, Swank RT, Seabra MC, Shivdasani RA. A role for Rab27b in NF-E2-dependent pathways of platelet formation. Blood. 2003 Dec 1;102(12):3970-9. Epub 2003 Aug 7. PubMed PMID: 12907454.

47: Barkalow KL, Falet H, Italiano JE Jr, van Vugt A, Carpenter CL, Schreiber AD, Hartwig JH. Role for phosphoinositide 3-kinase in Fc gamma RIIA-induced platelet shape change. Am J Physiol Cell Physiol. 2003 Oct;285(4):C797-805. Epub 2003 Jun 4. PubMed PMID: 12788695.

48: Italiano JE Jr, Bergmeier W, Tiwari S, Falet H, Hartwig JH, Hoffmeister KM, André P, Wagner DD, Shivdasani RA. Mechanisms and implications of platelet discoid shape. Blood. 2003 Jun 15;101(12):4789-96. Epub 2003 Feb 13. PubMed PMID: 12586623.

49: Italiano JE Jr, Shivdasani RA. Megakaryocytes and beyond: the birth of platelets. J Thromb Haemost. 2003 Jun;1(6):1174-82. Review. PubMed PMID: 12871316.

50: Barkalow KL, Italiano JE Jr, Chou DE, Matsuoka Y, Bennett V, Hartwig JH. Alpha-adducin dissociates from F-actin and spectrin during platelet activation. J Cell Biol. 2003 May 12;161(3):557-70. PubMed PMID: 12743105; PubMed Central PMCID: PMC2172952.

51: Falet H, Hoffmeister KM, Neujahr R, Italiano JE Jr, Stossel TP, Southwick FS, Hartwig JH. Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblasts. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16782-7. Epub 2002 Dec 3. PubMed PMID: 12464680; PubMed Central PMCID: PMC139221.

52: Schwer HD, Lecine P, Tiwari S, Italiano JE Jr, Hartwig JH, Shivdasani RA. A lineage-restricted and divergent beta-tubulin isoform is essential for the biogenesis, structure and function of blood platelets. Curr Biol. 2001 Apr 17;11(8):579-86. PubMed PMID: 11369202.

53: Italiano JE Jr, Stewart M, Roberts TM. How the assembly dynamics of the nematode major sperm protein generate amoeboid cell motility. Int Rev Cytol. 2001;202:1-34. Review. PubMed PMID: 11061562.

54: Lecine P, Italiano JE Jr, Kim SW, Villeval JL, Shivdasani RA. Hematopoietic-specific beta 1 tubulin participates in a pathway of platelet biogenesis dependent on the transcription factor NF-E2. Blood. 2000 Aug 15;96(4):1366-73. PubMed PMID: 10942379.

55: Italiano JE Jr, Lecine P, Shivdasani RA, Hartwig JH. Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes. J Cell Biol. 1999 Dec 13;147(6):1299-312. PubMed PMID: 10601342; PubMed Central PMCID: PMC2168104.

56: Italiano JE Jr, Stewart M, Roberts TM. Localized depolymerization of the major sperm protein cytoskeleton correlates with the forward movement of the cell body in the amoeboid movement of nematode sperm. J Cell Biol. 1999 Sep 6;146(5):1087-96. PubMed PMID: 10477761; PubMed Central PMCID: PMC2169480.

57: Stewart M, Roberts TM, Italiano JE Jr, King KL, Hammel R, Parathasathy G, Bullock TL, McCoy AJ, Kent H, Haaf A, Neuhaus D. Amoeboid motility without actin: insights into the molecular mechanism of locomotion using the major sperm protein (MSP) of nematodes. Biol Bull. 1998 Jun;194(3):342-3; discussion 343-4. PubMed PMID: 9664658.

58: Italiano JE Jr, Roberts TM, Stewart M, Fontana CA. Reconstitution in vitro of the motile apparatus from the amoeboid sperm of Ascaris shows that filament assembly and bundling move membranes. Cell. 1996 Jan 12;84(1):105-14. PubMed PMID: 8548814.